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Title:: SSR Identification of Soybean Line with Resistance to Both Soybean Mosaic? Virus and Phytophthpra Root Rot

Author(s):: -; ?（Key Laboratory of Soybean Biology in Chinese Ministry of Education，Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry,Northeast Agricultural University,Harbin 150030,China）

Keywords:: Soybean mosaic virus; Phytophthora root rot; Resistance; SSR identification

Abstract:: ?Soybean mosaic virus(SMV)and phytophthora root rot(PRR)are both worldwide soybean diseases,which seriously affect the yield and quality of soybean.Selecting resistance varieties is one of the most effective measures to control these two diseases.Traditional program for varieties with resistance to disease is timeconsuming,laborious and inefficient.SSR marker and molecularassisted breeding make effective selection of varieties with resistance to disease possible.A recombinant intercross line population including 146 F2∶〖KG-*2〗5 line was derived from a cross between F1(Dongnong93046×Hefeng25)× F1(Congrad×DongnongL5).SSR marker Satt114,identified by Teng et al(2005),and Satt325,Satt343,Satt428,Satt005,Satt600,Satt611,Satt689,Satt579,Satt252,Satt274 identified by Li et al(2010)were used to analyze these RI line with resistance to SMV and PRR.Moreover,phenotypic analysis for SMV and PRR was used to evaluate SSR identification.A total of 32 lines are resistance to SMV,28 of which are verified through friction inoculation test.The accuracy of molecular assistedbreeding is 87.5%.A total of 23 lines have 48 resistance loci to PRR,loss rate of which are 61.11%,47.08%,32.92%,26.11%,18.34%,respectively.In this study,four lines are highly resistant to both SMV and PRR.