The Impact Factors of Immature Cotyledons Transformation via Particle Bombardment in Soybean(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年06期
- Page:
- 735-739
- Research Field:
- Publishing date:
Info
- Title:
- The Impact Factors of Immature Cotyledons Transformation via Particle Bombardment in Soybean
- Author(s):
- -
- (1.School of Marine Science and Technology，Huaihai Institute of Technology，Lianyungang 222005，China;2.School of Environmental Science and Engineering，Tianjin University，Tianjin 300072，China;3.Agriculture and Parergon Base，Unit of 92323，PLA，Lianyungang 222247，China;4.Institute of Crop Science，Chinese Academy of Agricultural Sciences，Beijing 100081，China)
- Keywords:
- Soybean; Immature cotyledons; Somatic embryo; Particle bombardment; Genetic transformation
- PACS:
- -
- DOI:
- 10.11861/j.issn.1000-9841.2013.06.0735
- Abstract:
- Using immature cotyledons of soybean as explants，the effects of soybean genotype，pre-culture time of receptor before bombardment，bombardment times and target distance on transformation immature cotyledons of soybean via particle bombardment were investigated.The results showed that genotype of soybean had obvious effects on transformation of immature cotyledon.Rates of somatic embryogenesis in 40.91% of 22 genotypes used in the experiment reached more than 40% with at least 2 embryo.These genotypes were fitted as receptor for further transformation.Before Hefeng 25 was transformed by particle bombardment，treatment with 10 days pre-cultured was superior to those with 5 and 14 days pre-cultured treatment.Bombardment times did not affect on transformation.Effect of target distance on transformation varied with soybean genotypes.
Last Update: 2014-02-28