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Hemin-based Combinations of Exogenous Substances Alleviate the Inhibition of Soybean Seed Germination under Salt Stress(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2013年05期
Page:
640-646
Research Field:
Publishing date:

Info

Title:
Hemin-based Combinations of Exogenous Substances Alleviate the Inhibition of Soybean Seed Germination under Salt Stress
Author(s):
CHEN YunZHENG Huan-mingZHANG JingSHEN Wen-biao
?College of Life Science/Experiment Teaching Center of Biology,Nanjing Agricultural University,Nanjing 210095,China
Keywords:
SoybeanSalt stressSeed germinationGermination rateHeminAmylasePeroxidase
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2013.05.0640
Abstract:
?The aim of this study was to investigate a heminbased combination with other substances like CaCl2,salicylic acid(SA),proline(Pro)or KNO3 that could alleviate salt damage during soybean seed germination.In the experiment,soybean seeds of Sudou 5 from Jiangsu Academy of Agricultural Science,were used upon 150?mmol·L-1 NaCl.Subsequently,seed germination rate and index,the growth of radicle and hypocotyl of soybean,were measured and analyzed.The results showed that seed germination,index and the growth of radicle and hypocotyl of soybean were reduced under salt stress,while individually applied CaCl2,salicylic acid(SA),proline,KNO3 and hemin could all alleviate above salt damage.Furthermore,the mixture of hemin plus?CaCl2 or proline was more effective in alleviating salt toxicity.Meanwhile,both of them could enhance amylase and peroxidase(POD)activities.Together,these results clearly indicated that combinations of hemin and CaCl2 or proline exerted an advantageous effect on enhancing salt tolerance during soybean seed germination,for which 120 μmol·L-1 hemin together with 1.5 mmol·L-1mol CaCl2 and 80 μmol·L-1 hemin plus 10 mmol·L-1 proline were the best combinations.

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Last Update: 2013-11-12