Transformation of Soybean with Stress Related Gene DREB2A from Arabidopsis
(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年05期
- Page:
- 606-608
- Research Field:
- Publishing date:
Info
- Title:
- Transformation of Soybean with Stress Related Gene DREB2A from Arabidopsis
- Author(s):
- ?YU Zhi-jing; CAI Qin-an; LIU Yan-zhi; QI Guang-xun; MA Rui
- Keywords:
- Soybean; DREB2A; Salt and drought tolerance; Agrobacterium-mediated transformation
- PACS:
- -
- DOI:
- 10.11861/j.issn.1000-9841.2013.05.0606
- Abstract:
- ?To improve the salt and drought tolerance of soybean,a vector of pCAMBIA3300-DREB2A with stress related gene DREB2A from Arabidopsis thaliana was constructed. DREB2A gene was introduced into soybean varieties Williams 82 and Bert by Agrobacterium-mediated transformation.The average transformation frequencies were 0.8% and 3.2%，respectively.Twentyeight and 63 positive plants respectively were obtained by PCR identification.The results indicated that the gene DREB2A had been integrated into the genome of soybean.
Last Update: 2013-11-01