Abstract

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Author(s):: ?WANG Dong; SONG Jun; YE Xian-lin; LEI Shao-rong; LIU Wen-juan; CHANG Li-juan; YIN Quan; ZHANG Fu-li

Keywords:: Genetically modified soybean(GTS40-3-2); Content of NOS terminator; Measurement uncertainty; Evaluation

Abstract:: ?Uncertainty of Testing Content of NOS Terminator from Genetically Modified Soybean Samples by Realtime Quantitative PCR
A preliminary evaluation of measurement uncertainty of NOS terminator from transgenic soybean samples was made in this paper to understand the main contributors among sources of uncertainty and to further increase the quality of testing genetically modified organism(GMO)in future experiments.The measurement uncertainty estimates showed uncertainty of Type A(u_A),Type B(u_B)and combined uncertainty(u_C)was 0.002,0.002 and 0.003,respectively.When confidence level(P)was 95% and the coverage factor(k)was 1.96,the expanded uncertainty(U)was 0.016.Consequently,content of NOS terminator in this measurement was 0.032 ± 0.016,close to the conventional true value(0.03).The measurement uncertainty was relatively small,reflecting a high quality of detection.Measurement uncertainty of NOS terminator indicated the trace amount of liquid transfer was the major source of measurement uncertainty in this experiment.Thus,the trace amount of liquid transfer should be noted in future detection.
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