Abstract

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Title:: Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean

Author(s):: ?WANG Jun-wei¹; HOU Li-jiang¹; LI Deng¹; CHENG Chun-ming²; WANG Rui-zhen³; ZHAO Xian-wei³; ZHAO Chao-sen³

Keywords:: Wild soybean; Purple acid phosphotase; Low phosphorus tolerance gene; Resistance

Abstract:: ?In this study, PAP1 gene was cloned from wild soybean w343 planted under deficient Pi condition with designed primers according to the sequence of GmPAP1 gene from cultivated soybean based on homologous cloning method and named GsPAP1.The cloned GsPAP1 gene was 999 bp in size and encoded 332 amino acids as same as GmPAP1 gene from soybean,with molecular weight of 37.71 kD and isoelectric point 7.38.Sequence alignment of GmPAP1 withGsPAP1 showed that there were 9 single nucleotide polymorphisms,including 5 locus transitions and 4 locus transversion.GsPAP1 had higher homology to GmPAP1 with the identities of 99.38%.Amino acid sequence alignment showed that 7 deduced amino acids of GsPAP1 were different from GmPAP1. The phylogenic tree results showed that GsPAP1 gene from w343 shared high similarities to the previously reported PAP1 genes from other plants,such as Glycine max, ?Lupinus luteus, Ipomoea batatas, Vitis vinifera and so on.
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