Purity Identification,Molecular ID Establishment and Genetic Diversity Analysis of Soybeans Attending Heilongjiang Reclamation Regional Test in 2012(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年05期
- Page:
- 591-595
- Research Field:
- Publishing date:
Info
- Title:
- Purity Identification,Molecular ID Establishment and Genetic Diversity Analysis of Soybeans Attending Heilongjiang Reclamation Regional Test in 2012
- Author(s):
- ?HE Lin1; LIU Ye-li1; PEI Yu-feng2; LIU Chun-yan1; HAN Xue1; JIANG Hong-wei1; CHEN Qing-shan3; HU Guo-hua1
- Keywords:
- Soybean; Identification of purity; Molecular ID; Genetic diversity
- PACS:
- -
- DOI:
- 10.11861/j.issn.1000-9841.2013.05.0591
- Abstract:
- ?Simple sequence repeat(SSR)markers has proved to be effective in seed purity identification and genetic diversity analysis.In this paper,121 soybeans for regional test of Heilongjiang reclamation area in 2012 were used,their purity,molecular ID and genetic diversity were analyzed with 13 SSR primers distributed in 8 linkage groups of soybean genetic map.The purity of these varieties ranged from 84.62% to 100%,and averaged 99.24%.Ten SSR markers including Sat092,Satt138,Satt160,Satt231,Satt233,Satt288,Satt369,Satt442,Satt514 and Satt536,were used to identify the 121 soybeans,then a unique molecule ID was obtained for each line.Genetic similarity coefficients among these lines ranged from 0 to 1.00 and the mean coefficient was 0.340 2.The results indicate the soybeans attending regional test of Heilongjiang reclamation area in 2012 have smaller genetic homology.
Last Update: 2013-11-01