Comparison of Four Methods for Genomic DNA Extraction from? Aphis glycines(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年04期
- Page:
- 473-476
- Research Field:
- Publishing date:
Info
- Title:
- Comparison of Four Methods for Genomic DNA Extraction from? Aphis glycines
- Author(s):
- ?ZHANG Tuo; PANG Chun-jie; HAN Lan-lan; ZHAO Kui-jun
- ?College of Agriculture，Northeast Agricultural University，Harbin 150030，China
- Keywords:
- Aphis glycines; Genomic DNA; Extraction method
- PACS:
- S565.1
- DOI:
- 10.11861/j.issn.1000-9841.2013.04.0473
- Abstract:
- ?Using Aphis glycines as material，four genomic DNA extraction method including chloroform-isoamyl alcohol extraction，salting-out method，KAc and genomic DNA kit method were compared and analyzed. The purity of genomic DNA from chloroform-isoamyl alcohol extraction and salting-out method was better than the other two methods.And the total time of extraction in ascending order was genomic DNA kit method,salting-out method,the chloroform-isoamyl alcohol extraction and KAc.Salting-out method acted more quickly and effectively,the other three methods also had some advantages,therefore,the corresponding methods for genomic DNA extraction should be chosen according to the test conditions.
Last Update: 2014-02-17