Molecular Cloning,Sequence Analysis and Prokaryotic Expression of General Odorant Binding Protein 1 Gene from Agrotis c-nigrum(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年04期
- Page:
- 467-472
- Research Field:
- Publishing date:
Info
- Title:
- Molecular Cloning,Sequence Analysis and Prokaryotic Expression of General Odorant Binding Protein 1 Gene from Agrotis c-nigrum
- Author(s):
- ?WANG Shu1; HAN Lan-lan1; ZHAO Yin2; ZHAO Kui-jun1
- ?1.College of Agriculture,Northeast Agricultural University,Harbin 150030,China;2.Fuyu County Agricultural Technology Extension Center,Fuyu County 150030,China
- Keywords:
- Soybean; Agrotis c-nigrum; General odorant binding protein 1; Cloning; Prokaryotic expression; Sequence analysis
- PACS:
- S565.1
- DOI:
- 10.11861/j.issn.1000-9841.2013.04.0467
- Abstract:
- ?General odorant binding protein 1(GOBP1) is the important protein of insect chemosensory mechanism,and it has great significance for researching odor control of pest.In this test,a 1 260 bp gene fragment was cloned by using the technology of RTPCR with template of Agrotis cnigrum tentacles RNA(GenBank Accession Number:JX978464),which encoded 161 amino acid residues with 18.6 kD predicted molecular weight and pI 5.14.Online bioinformatics analysis showed nucleotide sequence homology of Agrotis c-nigrum GOBP1 with Agrotis segetum(Accession Number:DQ838577.1)reached 97%,and the similarity with other insects was higher than 80%.Western bolt detection showed it was the target protein.These results provide the basic data for the pest bio-control with odor control and the research of GOBP1.
Last Update: 2014-02-17