Construction and Indentification of Fulllength cDNA Library from Young Pod of Soybean Jinong 18(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年04期
- Page:
- 455-458
- Research Field:
- Publishing date:
Info
- Title:
- Construction and Indentification of Fulllength cDNA Library from Young Pod of Soybean Jinong 18
- Author(s):
- ?ZHOU Ying1; WANG Nan1; YIN Jun-qi1; YAO Dan2; MA Jian1; QU Jing1; WANG Pi-wu1
- ?1.College of Agronomy,Jilin Agricultural University,Changchun 130118,China;2.College of Life Sciences,Jilin Agricultural University,Changchun 130118,China
- Keywords:
- Soybean; Young pod; Fulllength cDNA library; SMART technology
- PACS:
- S336
- DOI:
- 10.11861/j.issn.1000-9841.2013.04.0455
- Abstract:
- ???? Construction of fulllength cDNA library from soybean young pod could lay foundation for cloning yield related genes.The total RNA from young pod of soybean Jinong 18 was separated and then transcribed into singlestrand cDNA,〖JP2〗which was synthesized into doublestrand cDNAs by LDPCR.Doublestrand cDNAs was digested by Proteinase K and Sfil,and fractionated by CHROMA SPIN400 columns.The ds cDNAs longer than 500 bp were collected and ligated to λTripIE×2,and then packaging reaction for recombinant bacteriophages was performed.The titer of the primary cDNA library was 1.5×106 pfu·mL-1,and the recombinant rate was 92%.The titer of amplified library was 2.6×108 pfu·mL-1and the insert size was more than 500 bp.Results showed that library titer and recombination rate met requirements of cDNA library,which would facilitate the functional genomics research of soybean.
Last Update: 2014-02-14