Effect of Hygromycin as a Screening Agent on the Induction of Soybean Hairy Roots(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年04期
- Page:
- 449-454
- Research Field:
- Publishing date:
Info
- Title:
- Effect of Hygromycin as a Screening Agent on the Induction of Soybean Hairy Roots
- Author(s):
- ?LIU Jing; LIU Jian-wei; HAN Tian-fu; HOU Wen-sheng
- ?Institute of Crop Science,Chinese Academy of Agricultural Sciences/MOA Key Laboratory of Soybean Biology(Beijing),Beijing 100081,China
- Keywords:
- Soybean; Genotype; Hairy root transformation technology; Hygromycin; Suitable concentration
- PACS:
- S565.1
- DOI:
- 10.11861/j.issn.1000-9841.2013.04.0449
- Abstract:
- ?? Selectable marker genes perform a fundamental and essential role in plant transformation by providing the mechanism for the selective survival,growth and development of transformed cells.In the research,four soybean genotypes were selected,and the effects of different concentrations of hygromycin on the hairy root induction and the root elongation were investigated,with the indicators of number of the hairy roots induced by cotyledons which were infected with the Agrobacterium rhizogenes and the root elongation separately.The purpose was to lay the foundation for the diversity of choices of the selection agent in the soybean genetic transformation process and for the hygromycin resistance breeding.The results showed that,the hygromycin could effectively inhibit the growth of nontransformed cells,successfully act as a successful screening agent in the soybean hairy root transformation system.However,a great difference among different soybean genotypes was exhibited in the sensitivity to hygromycin.The suitable screening concentrations of hygromycin for Zigongdongdou,Jilinxiaoli 1,Jiyu 47 and Zhonghuang 30 at the hairy root induction stage were 40,16,16,24 mg·L-1;and the suitable screening concentrations at the root elongation stage were 49,46,33,55 mg·L-1,respectively.
Last Update: 2014-02-14