Effect of Boron on Explant Regeneration and Frequency of Agrobacterium-mediated Transformation in Soybean(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年04期
- Page:
- 445-448
- Research Field:
- Publishing date:
Info
- Title:
- Effect of Boron on Explant Regeneration and Frequency of Agrobacterium-mediated Transformation in Soybean
- Author(s):
- ?HU Qian-qian; FANG Xing; WANG Jian-guo; YUE Na; MENG Xiang-xun
- ?School of Basic Medicine and Biological Science,Soochow University,Suzhou 215123,China
- Keywords:
- Soybean; Boron; Agrobacterium; GUS gene; Transformation
- PACS:
- S565.1
- DOI:
- 10.11861/j.issn.1000-9841.2013.04.0445
- Abstract:
- ???? Boron plays an important role in maintaining the structural and functional integrity of cell wall and cellularmembrane in plant.In the present experiment,the influence of concentration of boron element in the medium on the regeneration of cotyledonary node and induction of multiple shoots as well as the transformation efficiency of Agrobacteriummediated report gene(GUS)were investigated using seedlings of Jilinxiaoli 7 soybean as culture explant.The transient expression rates of GUS gene expression were calculated based on the histochemical method and the stable transformed shoots were further detected by PCR and Dot blotting to explore the efficiency of soybean transformation in a suitable boric acid concentration.The results showed that both high boron and low boron medium had an inhibition effect on induction of multiple shoots of cotyledonary node,especially at high level boron multiple shoots induction rates were significantly reduced.However,Agrobacterium-mediated transformation efficiency in soybean explant were significantly risen at 30 mg·L-1boric acid concentration compared with normal boric acid environment,with the GUS transient conversion rate as high as 35.92%.The significance of this phenomenon remains to be further explored for establishment of a new soybean genetic transformation system.
Last Update: 2014-02-14