Technology of RNAi and Its Application in Genetic Engineering against Soybean Cyst Nematode of Soybean(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年04期
- Page:
- 548-554
- Research Field:
- Publishing date:
Info
- Title:
- Technology of RNAi and Its Application in Genetic Engineering against Soybean Cyst Nematode of Soybean
- Author(s):
- ?YANG Qiao; LIU Qian; JIAN Heng
- ?Department of Plant Pathology,China Agricultural University,Beijing 100193,China
- Keywords:
- RNA interference; Resistance to soybean cyst nematode; Genetic engineering
- PACS:
- S565.1
- DOI:
- 10.11861/j.issn.1000-9841.2013.04.0548
- Abstract:
- ?Soybean production losses caused by soybean cyst nematode(Heteterodera glycines Ichnohe)are increasing continually.Traditional control strategies such as rotation system and nematicides can’t meet the needs of modern agriculture development.Resistance breeding and transgenic breeding also have serious limitations.Recently RNA interference technology represents an emerging breakthrough in the application of functional genomics for genetic engineering against soybean cyst nematode.Screen for potential target genes by the application of bioinformatics,and then synthesize dsRNAs or construct the RNAi vector carrying the desired gene sequences.dsRNA or siRNAs of essential genes was oral uptaked by established plant parasitic nematodes through the feeding tube,which elicit a systemic RNAi response and then induce a highly detrimental(suppressing nematode parasitism,development,metabolism,motion,and reproduction)or even lethal RNAi phenotype in nematodes.Although there is still poor understanding of the range of factors(dsRNA concentrations,construct size,et al)that influence RNAi phenotype,modifying current RNAi strategies and performing proper ecological risk assessment can also highlight the novel strategies available for revealing gene function of nematodes and improving host derived RNAi resistance.In the near future,small RNA sequencing techniques and amiRNAi can promote the development of RNAi technologies.This paper reviews the mechanism of RNAi against plant parasitic nematodes,the progress of RNAi-mediated defense against soybean cyst nematodes,the characteristics and some key points of this strategy,and also prospect of its application.
Last Update: 2014-04-04