Protective Effects of Soy Isoflavones on Acute Liver Injury Induced by Lipopolysaccharide and D-galactosamine in Mice(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年04期
- Page:
- 544-547
- Research Field:
- Publishing date:
Info
- Title:
- Protective Effects of Soy Isoflavones on Acute Liver Injury Induced by Lipopolysaccharide and D-galactosamine in Mice
- Author(s):
- ?YIN Xue-zhe1; JIN Yan-hua1; JIN Mei-hua2; JIN Ming2
- ?1.Affiliated Hospital of Yanbian University,Yanji 133000,China;2.Medical College of Yanbian University,Yanji 133000,China
- Keywords:
- Soybean isoflavones; Liver injury; Anti-inflammatory; Anti-apoptotic
- PACS:
- %151.41
- DOI:
- 10.11861/j.issn.1000-9841.2013.04.0544
- Abstract:
- ?For the purpose of studying the protective effect of soy isoflavones on acute liver injury induced by D-galactosamine(GalN)and lipopolysaccharide(LPS)in mice,the tested mice were randomly assigned to 5 groups:normal control,model control,silymarin(positive control),as well as high and low dose groups of soy isoflavones.Animals were treated once daily for 7 days.GalN and LPS were given intraperitoneally to the mice of groups,then the pathological changes was detected with hematoxylin & eosin(HE)staining,tumor necrosis factor-α(TNF-α)was detected by ELISA method,and the alanine aminotransferase(ALT),aspartate aminotransferase(AST),lipid hydroperoxide(LOOH),malondialdehyde(MDA),nitric oxide(NO),and the activation of Caspase-3 and Caspase-8 were detected by the colorimetric method.The results showed that the soy isoflavones reduced the serum ALT and AST,alleviated the hepatic lesions induced by GalN and LPS,decreased serum TNF-α and hepatic NO levels,reduced the hepatic LOOH and MDA contents,and decreased the Caspase-3 and Caspase-8 activations of liver tissues.It is suggested that soy isoflavones have protective effect on acute liver injury induced by GalN and LPS in mice,probably via the anti-inflammatory,antioxidative and anti-apoptotic ability.
Last Update: 2014-04-04