Optimization on the Purification Technologies of Soybean Isoflavone(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年04期
- Page:
- 535-538
- Research Field:
- Publishing date:
Info
- Title:
- Optimization on the Purification Technologies of Soybean Isoflavone
- Author(s):
- ?LIU Shao-jing1; WANG Duo-ning2; DIAO Ying-bo1; XIAO Qiu-ru1; ZHANG Yu-qi1; YANG Li-bin1
- ?1.College of Pharmacy,Xi’an Medical University,Xi’an 710021,China;2.Scientific Research Center,Xi’an Medical University,Xi’an 710021,China
- Keywords:
- Soybean meal; Soybean isoflavone; Purification; Ethanol precipitation; Extraction
- PACS:
- TS202.3
- DOI:
- 10.11861/j.issn.1000-9841.2013.04.0535
- Abstract:
- ?With the purity of soybean isoflavone as investigation index,the isoflavone purification method of alcohol precipitation combined with ethyl acetate extraction was compared with other methods.And then the alcohol precipitation parameters for the purification of isoflavones were optimized by single-factor and orthogonal experiment.The optimized conditions of ethanol precipitation were,mixing 25% mass fraction of isoflavone extract with 80% ethanol for 13 min,and then standing for 5 h;After 3 times extraction with ethyl acetate,isoflavones could be extracted completely.Under the new technology of alcohol precipitation and ethyl acetate extraction,the purification of isoflavones in final product was 16.83%,increased nearly 4 times than separate ethanol precipitation.
Last Update: 2014-04-04