A Rapid Method for Determination of Soybean Isoflavone Components in Feeds by HPLC(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年04期
- Page:
- 530-534
- Research Field:
- Publishing date:
Info
- Title:
- A Rapid Method for Determination of Soybean Isoflavone Components in Feeds by HPLC
- Author(s):
- ?ZHANG Jing-ying; SUN Jun-ming; LI Bin; HAN Fen-xia; YAN Shu-rong; TIAN Ling; CHEN Ming-yang; ZOU Xiao; FAN Sheng-xu; YANG Hua
- ?Institute of Crop Science,Chinese Academy of Agricultural Sciences/Ministry of Agriculture(MOA)Key Laboratory of Soybean Biology,Beijing 100081,China
- Keywords:
- Soybean; Feed; Isoflavone; HPLC
- PACS:
- S565.1
- DOI:
- 10.11861/j.issn.1000-9841.2013.04.0530
- Abstract:
- ?Isoflavone is a kind of new feed additives with enhancing immunity,improving animal reproduction,secretion ability and other physiological functions.However,the unreasonable dose will cause potential side effect.In this study,we extracted the isoflavone with 0.1%(V/V)acetic acid 70%(V/V)ethanol solution from nine kinds of feeds,and identified the isoflavone components by high-performance liquid chromatography(HPLC).The results showed that the method could quickly detect all the isoflavone components except for acetylglycitin in feeds.Comparing to the soybean seed,the malonyl-glucoside groups of isoflavone were less,the free aglycone groups were increased correspondingly and the acetylgenistin content was higher in feeds.Owing to the various formulas and materials,there was significant difference in the isoflavone content in various kinds of feeds.Based on the ANOVA analysis,the method presented high accuracy and stability.It can be used to rapidly detect the isoflavones content in feeds and to provide guidance for livestock and poultry production.
Last Update: 2014-04-04