Genetic Diversity and Phylogeny of Soybean Rhizobia Isolated from Northeast China(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年04期
- Page:
- 433-437
- Research Field:
- Publishing date:
Info
- Title:
- Genetic Diversity and Phylogeny of Soybean Rhizobia Isolated from Northeast China
- Author(s):
- WANG Wei-wei; GUAN Da-wei; MA Ming-chao; LI Li; CAO Feng-ming; LI Jun
- Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences/Key Laboratory of Microbial Resources Collection and Preservation,Ministry of Agriculture,Beijing 10081,China
- Keywords:
- Soybean rhizobia; BOXPCR; IGS PCRRFLP; 16S PCRRFLP; Genetic diversity; Phylogeny
- PACS:
- S514
- DOI:
- 10.11861/j.issn.1000-9841.2013.04.0433
- Abstract:
- In order to reveal the genetic diversity and phylogeny of soybean rhizobia in the Northeast China,BOX-PCR，16S rDNA PCR-RFLP，IGS PCRRFLP and 16S rDNA gene sequencing methods were used to analyze 312 soybean rhizobia isolated from 18 soybean cultivars in 14 sites in the Northeast China.The results indicated that all the tested rhizobia belonged to Bradyrhizobium,of which B.japonicum was the dominant species.Under IGS PCRRFLP 72% and 16S rDNA PCR-RFLP 76% similarity level,tested rhizobia were divided into 6 and 4 groups,respectively.BOX-PCR fingerprint showed the tested rhizobia were divided into 31 groups under 90% similarity level,which suggested the abundance genetic diversity of Bradyrhizobium in this area.The results suggested that the genetic diversity of tested strains was closely related to the geographical environment,rather than their soybean cultivar hosts.
Last Update: 2014-02-14