Preliminary Studies on the Expression Pattern of Soybean P39-1 Gene and Promoter(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年03期
- Page:
- 425-428
- Research Field:
- Publishing date:
Info
- Title:
- Preliminary Studies on the Expression Pattern of Soybean P39-1 Gene and Promoter
- Author(s):
- ZHAO Yan1; ZHAO Dan2; LIU Bo1; GUO Chun-hui1
- (1.College of Life Science and Agroforestry,Qiqihar University,Qiqihar 161006,China;2.Jilin City No.7 Middle School,Jilin 132011,China)
- Keywords:
- Soybean; P39-1 gene; Promoter; Cloning
- PACS:
- S565.1
- DOI:
- 10.11861/j.issn.1000-9841.2013.03.0425
- Abstract:
- The expression pattern of soybean P39-1 gene in soybean tissues was detected by real-time quantitative PCR(RTQ-PCR),and the result showed that it had lower activity in roots,stems and leaves,while higher activity in flowers and seeds.The 5’flanking upstream sequence of soybean P39-1 gene,named P39-1-p,was isolated from soybean genomic DNA by PCR method,and the length was 2 000 bp.Sequence analysis revealed that this fragment contained a series of motifs related to seed specific promoters and some pollenexpressed elements,such as SEF1 motif,SEF3motif,SEF4 motif,E-box,G-box,AACACA,AACA,ACGT,CCAA；52-box,ntp303-box,GTGA and TACPyATbox.It is inferred that P39-1-p promoter possesses the function driven downstream gene expression abundantly in soybean flowers and seeds.
Last Update: 2014-03-04