Separation and Properties of Urease in Sword Bean and Black Soybean(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年03期
- Page:
- 361-364
- Research Field:
- Publishing date:
Info
- Title:
- Separation and Properties of Urease in Sword Bean and Black Soybean
- Author(s):
- ZHANG Kuan-chao; RUAN Fei; JIN Qing; WEN Han
- (School of Life Science,Anhui Agricultural University,Hefei 230036,China)
- Keywords:
- Sword bean; Black soybean; Urease; Separation; Property
- PACS:
- S565.1
- DOI:
- 10.11861/j.issn.1000-9841.2013.03.0361
- Abstract:
- In order to explore the urease in leguminaceous plant,the purification and properties of urease in sword bean(Canavalina sp.)and black soybean(Glyeine soja Sieb.et Zucc)were studied.Urease were isolated and purified by mineral ether-degrease,cold acetone deposition,sulfate grade deposition,dialyseion and exchange chromatography.Purification multiple of ureases from sword bean and black soybea was 3.851 and 3.36,recovery was 5.35% and 11.27%.The chart of ureases reaction showed that the output and reaction time was linear in 15 min.The Km values of sword bean and black soybea were 1.3×10-2 and 2.4×10-3 mol·L-1,while the optimum pH were 7.0 and 6.0,and the pH stability were 6.0-7.0 and 5.0-8.0.
Last Update: 2014-03-04