Tap Water Can be Used as Medium for Root Induction and Growth during Tissue Culture of Soybean(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年03期
- Page:
- 333-335
- Research Field:
- Publishing date:
Info
- Title:
- Tap Water Can be Used as Medium for Root Induction and Growth during Tissue Culture of Soybean
- Author(s):
- ?HU Feng; YANG Wan-nian
- ?College of Life Sciences,Central China Normal University,Wuhan 430079,China
- Keywords:
- Soybean; Rooting in water culture; Growth rate:Survival rate
- PACS:
- S565.1
- DOI:
- 10.11861/j.issn.1000-9841.2013.03.0333
- Abstract:
- ?Rootinducing is the final step for producing regenerated plantlets in the tissue culture of soybean\[Glycine max(L.)Merr.\].Here an easy,efficient rootinducing system for soybean shoots is reported,which is designated as tapwater rooting system,in which tap water is(without autoclaving)used to replace the traditional agarcontaining medium for root induction and growth.Taking Zigongdongdou as material,the results demonstrated that the tapwater rooting system was good for shortening culture time,overcoming contamination of bacterium and fungus,increasing the rooting efficiency and decreasing the cost of tissue culture as exemplified by faster root inducing and elongating,and higher plantlet survive ratio of the tap-water system than that of agar-containing medium system.Although the amount of total roots from the tapwater system was less than that from agar plates,the plantlets from tapwater system could grow,flower and produce pods and seeds normally and no significant difference were found between the tapwater system and agar system.Hence,it is suggested that the easy rooting system should be used in the tissue culture of more soybean cultivar.
Last Update: 2014-03-04