Construction of RNAi Expressed Vector of Soybean Agglutinin le2 Gene and Transform Research(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年03期
- Page:
- 306-309
- Research Field:
- Publishing date:
Info
- Title:
- Construction of RNAi Expressed Vector of Soybean Agglutinin le2 Gene and Transform Research
- Author(s):
- ?WEI Qiang; LI Lu-hua; WANG Chun-yan; FU Yong-ping; MA Jian; QU Jing; WANG Pi-wu
- Agronomy College of Jilin Agricultural University，Changchun 130118,China
- Keywords:
- RNAi; Soybean agglutinin; Construction of vector; Transform
- PACS:
- S565.1
- DOI:
- 10.11861/j.issn.1000-9841.2013.03.0306
- Abstract:
- ?For the purpose of constructing RNAi expressed vector,and discussing the method to improve soybean quality,according to known conserved sequence(AY342212)of soybean agglutinin le2 gene in GenBank,right primer was designed and the conserved sequence was cloned.And the homology was 99.77% contrast to the original sequence.Herein,using P3301-PFNA-α’ as the basic vector,the expression vector pCAMBIA3301-le2RNAi was constructed which contained RNA interference vector and bar gene through subcolone.The RNA interference expression vector was then transformed into cotyledon nodes of soybean Jinong 28 by Agrobacterium mediated method.5 positive transgenic plants in T1 generation were confirmed by PCR detection and 23 seeds were harvested from T1 transgenic plants.The results provided a basis for soybean quality improvement.
Last Update: 2014-03-04