Genetic Transformation and Function Analysis of Transcription Factor GmPHR1 in Soybean(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年03期
- Page:
- 302-305
- Research Field:
- Publishing date:
Info
- Title:
- Genetic Transformation and Function Analysis of Transcription Factor GmPHR1 in Soybean
- Author(s):
- ?WU Bing; LI Xi-huan; LIU Cui; WANG Yun-jie; LI Wen-long; CHANG Wen-suo; ZHANG Cai-ying
- ?North China Key Laboratory of Crop Germplasm Resources,Education Ministry of China/Agricultural University of Hebei,Baoding 071001,China
- Keywords:
- Soybean; MYB transcription factor; Vector construction; Agrobacterium mediated transformation; Highly expressed
- PACS:
- S565.1
- DOI:
- 10.11861/j.issn.1000-9841.2013.03.0302
- Abstract:
- ?To develop new transgenic soybean materials with MYB transcription factor GmPHR1, and to study the expression patterns of GmPHR1 in different low P tolerant soybean varieties,a over expression vector pBI121 GmPHR1 was constructed and introduced into Jidou12 by Agrobacterium mediated cotyledonarynode transformation method.PCR and realtime quantitative PCR analysis results of the transformed T4 plants showed that the GmPHR1 was successfully incorporated into the soybean genome and expressed at a higher level than the wild-type.Expression patterns assayed by real-time quantitative PCR revealed that the expression level of GmPHR1 in low P tolerant variety NF37 was induced quickly for a longer period at a higher level than that in sensitive variety Niumaohuang under phosphate starvation condition.
Last Update: 2014-03-04