Abstract

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Title:: Identification of Resistance Genes to Pythium Species in Soybeans by Association Analysis

Author(s):: ?WEI Lai1; 4; XUE Yong-guo1; WANG Wei-wei2; LIU Li-jun1; LI Wen-bin3; YU De-yue4; NAN Di1; LIANG Hui-xin1; LOU Bing-gan5; ?1.Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;2.Agricultural Products Quality and Safety Research Institute,Heilongjang Academy of Agricultural Sciences,Harbin 150086,China；3.Soybean Research Institute of Northeast Agricultural University，Harbin 150030,China;4.Soybean Research Institute of Nanjing Agricultural University,Nanjing 210095,China;5.Institute of Biotechnology,Zhejiang University,Hangzhou 310029,China

Abstract:: ?By using 150 soybean varieties with different ecotype as materials,and 4 Pythium species as inoculation pathogens,the cultivars resistance were identified through measuring the root rot level.256 SSR markers were used to analysis the population structure and linkage disequilibrium.A soybean resistance association analysis to 4 Pythium species was run with GLM procedure from software TASSEL,and a T test on associated markers with significant difference was carried on,to uncover associated loci conferring effects.The result showed:there was a comprehensive linkage disequilibrium;the collinear locus D′declined quickly,which made it fit for LD analysis;tested cultivars were divided into seven subgroups by the structural analysis of SSR data.There were 25 markers obtained by association analysis,which were connected with the resistance to 4 Pythium species,respectively;12 association loci with significant difference,were found confering major effect values to the tested cultivars through T test and resistance identifying.The loci with significant difference highly matched the cultivars’ resistant testing results;especially the ones with high reliability in association analysis showed the same on T test,such as the loci associated with P.aphanidermatum resistance,Satt191-1,Satt584-1,Satt584-2,and those with P.irregular resistance,Satt602-1,Satt042-4.