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Optimization on Cotyledonarynode Genetic Transformation System of Soybean Cultivar Jilin 47(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2013年03期
Page:
291-294
Research Field:
Publishing date:

Info

Title:
Optimization on Cotyledonarynode Genetic Transformation System of Soybean Cultivar Jilin 47
Author(s):
?SUN Xin; ZHAO Jian-ru; YAN Fan; JI Chang-yuan; YANG Xu-guang; LI Jing-wen; WANG Ying; WANG Qing-yu
?Plant Science College of Jilin University,Changchun 130062,China
Keywords:
Jilin 47; Cotyledonarynode; Browning; Shoot elongation
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2013.03.0291
Abstract:
?After infected by EHA105 caring plasmid pCAMBIA1301,the GUS staining of 10 soybean genotypes was carried out to detect the sensitivity for Agrobacterium tumefaciens.Jilin 47，Jilin 35 and Suinong 10 were screened out as sensitive genenotypes.Jilin 47 was chosen as the genotype for optimization of soybean cotyledonary genetic transformation due to its good performance on shoot regeneration.Amending thiol compounds in liquid and solid cocultivation medium could decrease the browning rate of explants and the best combination of 400?mg·L-1 L-Cys, 248 mg·L-1 sodium thiosulfate and 300 mg·L-1DTT were obtained through orthogonal test.The highest elongation rate was got when 1.0 mg·L-1 GA3 and 0.5 mg·L-1IAA were added to shoot elongation medium.

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Last Update: 2014-03-04