Analysis on Resistance to Frogeye Leaf Spot between Two Species of Transgenic Soybean Progenies with HrpZpsta Gene(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年02期
- Page:
- 238-241
- Research Field:
- Publishing date:
Info
- Title:
- Analysis on Resistance to Frogeye Leaf Spot between Two Species of Transgenic Soybean Progenies with HrpZpsta Gene
- Author(s):
- YIN Jun-qi; WANG Nan; ZHOU Ying; LU Shi; WU Nan; QU Jing; ZHANG Zhuo; WANG Pi-wu
- （College of Agronomy,Jilin Agricultural University，Changchun 130118,China）
- Keywords:
- Soybean Cercospora sojina; hrpZpsta gene; Screening for disease resistance; Evaluation of resistance
- PACS:
- -
- DOI:
- 10.3969/j.issn.1000-9841.2013.02.022
- Abstract:
- Soybean Cercospora sojina is caused by fungal pathogens,which lead to plant death.Protein harpin which coded by gene hrpZpsta has been proved to elevate resistance for plants against several kinds of fungal pathogen by hypersensitive reaction.The recombinant expressed vector which contained hrpZpsta gene had been transformed into two soybean cultivars,Jinong 17 and Jinong 29,and T4 transgenic plants were identified both in molecular way and artificial inoculations.Southern blot and RTPCR test showed that hrpZpsta gene could inherit in T4 transgenic plants steadily on the molecular level,and artificial inoculation test revealed the resistant ability to Cercospora sojina had been improved obviously.In addition,the resistance to frogeye leaf spot varied with soybean cultivars.
Last Update: 2014-04-03