Kanamycin and NaCl Tolerance of Adventitious Shoot Induced from Hypocotyl in Soybean(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年02期
- Page:
- 211-215
- Research Field:
- Publishing date:
Info
- Title:
- Kanamycin and NaCl Tolerance of Adventitious Shoot Induced from Hypocotyl in Soybean
- Author(s):
- GUO Qiu-yun1; 2; WANG Ping1; 2; LIU Zhao-pu1
- (1.Key Laboratory of Marine Biology of Jiangsu Province/College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095,China;2.Jiangsu Key Laboratory of Marine Biotechnology/School of Marine Science and Technology,Huaihai Institute of Technology,Lianyungang 222005,China)
- Keywords:
- Soybean; Hypocotyl; Kanamycin; NaCl; Adventitious shoot
- PACS:
- -
- DOI:
- 10.3969/j.issn.1000-9841.2013.02.016
- Abstract:
- In order to discuss the tolerance of different genotypes of soybean varieties to kanamycin and NaCl,hypocotyl from soybean genotypes including Heinong 44,Hefeng 35 and Huaidou 9 were used as the explants to investigate the formation of adventitious shoot under stress with kanamycin and NaCl.The results showed that the tolerance to kanamycin and NaCl varied with genotypes involved.With the increasing of kanamycin and NaCl concentration,the induction rate of adventitious shoot,number of shoot and length of shoot were significantly decreased in three genotypes.The critical concentration of kanamycin for Heinong 44,Hefeng 35 and Huaidou 9 were 100,150 and 100 mg·L-1 and that of NaCl for three genotypes were 100,100 and 75 mmol·L-1,respectively.
Last Update: 2014-04-03