Construction of Fusion Gene Expression cDNA Library of Soybean Root with Yeast One-hybrid Method(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年02期
- Page:
- 165-167
- Research Field:
- Publishing date:
Info
- Title:
- Construction of Fusion Gene Expression cDNA Library of Soybean Root with Yeast One-hybrid Method
- Author(s):
- LIU Qing-xing1; GUO Chang-hong1; BI Ying-dong2; GUO Dong-lin1
- Keywords:
- -
- PACS:
- -
- DOI:
- 10.3969/j.issn.1000-9841.2013.02.006
- Abstract:
- Gene sequence of protein which interacted with specific cis-element can be found by screening cDNA library in yeast one-hybrid system.Yeast one-hybrid system has become an important efficient method to investigate DNA-protein interaction and function of transcription factor.In this report,gene expression cDNA library fused to activation domain of Gal4p was constructed.Library was investigated using W-box cis-element in yeast one-hybrid system.As a result,4 positive clones were found.The study paved a foundation for screening WRKY protein which interacted with W-box cis-element.
Last Update: 2014-01-23