Isolation and Analysis of Promoter of GmGAMYB1 from Soybean(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年02期
- Page:
- 0-
- Research Field:
- Publishing date:
Info
- Title:
- Isolation and Analysis of Promoter of GmGAMYB1 from Soybean
- Author(s):
- GU Yue-jiao; ZHAO Lin; ZHANG Yan-wei; LU Qing-yao; SONG Xian-ping; LI Wen-bin
- Keywords:
- ; GmGAMYB1 ; promoter; GUS gene; Histochemical staining
- PACS:
- -
- DOI:
- 10.3969/j.issn.1000-9841.2013.02.005
- Abstract:
- Soybean(Glycine max)is a kind of typical plant in the developmental biology and molecular biology research,the completion of soybean genome has provided a lot of convenience for research,but there is still so little achievement on the soybean highly effective inducible promoters.Gibberellin(GA) biosynthesis and signaling pathways have been discussed in detail in recent years.In addition,several reviews discuss general mechanisms of morphogenesis.GAMYB1 gene is an important promotive factor in the signal transduction pathways of GA.In this study,the promoter of GmGAMYB1 was cloned from the soybean Dongnong 42 by PCR.It was directionally replaced CAMV 35S constitutive promoter of the vector pBI121.The expression vector of pBI121pGmGAMYB1 was constructed and the expression of downstream reporter GUS gene was drived.The analysis on promoter sequence by online promoter forecasting tool PLACE and PlantCARE showed that sequence contained a variety of specific expression elements,such as light and various hormones and stressinduced elements.GUS histochemical staining was used to explore the role of the promoter in plant growth regulation.This study had laid a good foundation for GmGAMYB1 characteristics temporal and spatial expression.
Last Update: 2014-01-23