Genetic Transformation of Transcription Factor AtPHR1 into Soybean by Agrobacterium-Mediated Method(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年02期
- Page:
- 154-159
- Research Field:
- Publishing date:
Info
- Title:
- Genetic Transformation of Transcription Factor AtPHR1 into Soybean by Agrobacterium-Mediated Method
- Author(s):
- ZHAO Li-li; LI Xi-huan; LI Wen-long; CHANG Wen-suo; ZHANG Cai-ying
- Keywords:
- MYB transcription factor; Agrobacterium-mediated method; Soybean; Genetic transformation
- PACS:
- -
- DOI:
- 10.3969/j.issn.1000-9841.2013.02.004
- Abstract:
- In this study，a MYB transcription factor AtPHR1 was introduced into soybean varieties by Agrobacterium-mediated cotyledonary node transformation method to study the influence factors on transformation efficiency.The studied factors included seedling age，preincubation period，strain density，infectious concentration，kanamycin concentration and soybean genotype.The results showed that a much higher induction rate of resistant shoots was obtained when the seedling age was 5 d from germination with 1 d pre-culture，the strain density(OD600)was 0.7 and infectious concentration was 0.5 or 0.7，and also the kanamycin concentration was 100 mg·L-1.The soybean variety，Wuxing 2，with the highest transformation efficiency was about 2.0%.Finally，five new PCR positive transgenic materials with transcription factor AtPHR1 were gained.
Last Update: 2014-01-23