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Effect of Knamycin and Glufosinate on Adventitious Buds Induction from Embryonic Tip of Different Genotype Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2013年01期
Page:
136-138
Research Field:
Publishing date:

Info

Title:
Effect of Knamycin and Glufosinate on Adventitious Buds Induction from Embryonic Tip of Different Genotype Soybean
Author(s):
ZHANG Xin-shuang1; 2; WANG Ping1; 2; SONG Hai-xing1; RONG Xiang-min1
1.College of Resources and Environment,Hunan Agricultural University,Changsha 410128,Hunan;
2.School of Marine Science and Technology,Huaihai Institute of Technology,Jiangsu Key Laboratory of Marine Technology,Lianyungang 222005,Jiangsu,China
Keywords:
Soybean; Embryonic tips; Adventitious bud; Knamycin; Glufosinate
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2013.01.032
Abstract:
Embryonic tips of Hefeng35,Heinong44 and Jilin35 were used as the explants to investigate the effects of kanamycin and glufosinate on the adventitious bud induction.Under 100 mg·L-1 kanamycin,the adventitious bud induction rate of Hefeng35 and Jilin35 were not significantly different compared with control,while Heinong44 was significantly lower than control;bud number of Jilin35 was lower than control and higher than other concentrations,Hefeng35 and Heinong44 were not different compared with control;the shoot length of 3 genotypes were significantly inhibited by 100 mg·L-1 kanamycin.Glufosinate at the concentration from 0.2 to 1.2 mg·L-1had no influence on the number of adventitious buds for Hefeng35 and Jilin35,but Heinong44 reduced conspicuously at the concentration of 1.0 mg·L-1.Both Heinong 44 and Jilin 35 were inhibited by 0.2 mg·L-1glufosinate at length of buds while Hefeng35 was inhibited by 0.6 mg·L-1 glufosinate.Results suggest the suitable screening concentration of kanamycin for Hefeng35,Heinong44 and Jilin35 were 100 mg·L-1,and glufosinate for three genotypes were 0.6,0.2 and 0.2 mg·L-1,respectively.

References:

［1］Olhoft P M,Lex E,Lagel F,et al.Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method[J].Planta,2003,216(5):723-735.

[2]Aragao F J L,Sarokin L,Vianna G R,et al .Selection of transgenic meristematic cells utilizing a herbicidal molecule results in the recovery of fertile transgenic soybean plants at high frequency[J].Theoretical and Applied Genetics,2000,101:1-6.
[3]Rech E L,Vianna G R,Aragao F J L.High-efficiency transformation by biolistics of soybean,common bean and cotton transgenic plants[J].Nature Protocols,2008,3(3):410-418.
[4]Zeng P,Vadnais D A,Zhang Z,et al.Refined glufosinate selection in Agrobacterium mediated transformation of soybean[Glycine max(L.)Merrill][J].Plant Cell Reports,2004,22:478-482.
[5]王伟,王罡,季静,等.大豆胚尖再生体系的优化及与子叶节再生体系的比较[J].大豆科学,2012,31(3):353-357.(Wang W,Wang G,Ji J,et al.Optimization of embryonic tip regeneration system and comparison with cotyledonary node regeneration system in soybean[J].Soybean Science,2012,31(3):353-357.)
[6]王萍,张淑珍,李文滨,等.大豆不同基因型胚尖不定芽的诱导及对抗生素的敏感性[J].作物杂志,2010(2):50-53.(Wang P,Zhang S Z,Li W B,et al.Induction of adventitious shoots from embryonic tip of different soybean genotypes and their sensibility to antibiotics[J].Crops,2010(2):50-53.)
[7]Komatsuda T,Ohyama K.Genotypes of high competence for somatic embryogenesis and plant regeneration in soybean Glycine max[J].Theoretical Applied Genetics,1988,75:695-700.
[8]王萍,管娟娟,冯远航,等.草铵膦对大豆胚尖不定芽形成的影响[J].作物杂志,2011(6):60-62.(Wang P,Guan J J,Feng Y H,et al.Effects of glufosinate on formation of adventitious buds from embryonic tip of soybean[J].Crops,2011(6):60-62.)
[9]Hakeem K R,Khan F,Chandna R,et al.Genotypic variability among soybean genotypes under NaCl stress and proteome analysis of salt-tolerant genotype[J].Applied Biochemistry and Biotechnology,2012,168(8):2309-2329.
[10]王萍,吴颖,季静,等.抗生素对大豆愈伤组织的诱导和生长的影响[J].遗传,2001,23(4):321-324.(Wang P,Wu Y,Ji J,et al.Effect of antibioties on induction of callus and callus growth in soybean[J].Hereditas,2001,23(4):321-324.)

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Last Update: 2013-02-20