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Pathogenicity of Entomopathogenic Nematode on Xestia c-nigrum in Soybean Field(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2013年01期
Page:
63-71
Research Field:
Publishing date:

Info

Title:
Pathogenicity of Entomopathogenic Nematode on Xestia c-nigrum in Soybean Field
Author(s):
ZHANG Si-jia12QIAN Xiu-juan3LI Chun-jie1PAN Feng-juan1XU Yan-li1
1.Key Laboratory of Malisons Agroecology,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150081,Heilongjiang;
2.Graduate University of Chinese Academy of Sciences,Beijing 100049;
3.Pratacultural College,Gansu Agricultural University,Lanzhou 730070,Gansu,China
Keywords:
Entomopathogenic nematode Xestia c-nigrumBioassayPathogenicity
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2013.01.015
Abstract:
Xestia c-nigrum L.is one of the pests seriously damaging soybean and other agricultural crops.In order to achieve entomopathogenic nematode with higher pathogenicity and optimum control stage of Xestia c-nigrum,four strains of Steinernema carpocapsae and three strains of Heterorhabditis bacteriophora were used to test pathogenicity against juvenile 3 and 5 of Xestia c-nigrum on filter paper.The results showed that seven strains of entomopathogenic nematode had pathogenicity on juvenile 3 of Xestia c-nigrum,and the pathogenicity of Steinernema carpocapsae was stronger than that of? S.glaseri and Heterorhabditis bacteriophora. Steinernema carpocapsae- 2(Sc-2)had the fastest lethal speed on juvenile 3 of Xestia c-nigrum;the half lethal time was 36 h,and the half lethal time of other strains of entomopathogenic nematode was longer than 48 h.The correct cumulative mortality of juvenile 3 infected by Sc-2 was higher than that caused by other nematode stains when juvenile 3 was infected for 24,36 and 72 h,and the correct cumulative mortality reached at 100% when juvenile 3 was infected for 96 h.The correct cumulative mortality of juvenile 5 infected by Sc-2 was higher than that caused by other nematode strains when juvenile 5 was infected for 24,36,72,96 and 120 h.The pathogenicity of Sc-2 was the strongest on Xestia c-nigrum among the seven nematode strains.The pathogenicity of Sc-2 decreased as Xestia c-nigrum grew up,and the correct mortality of juvenile 3 was higher than that of juvenile 5 when they were infected by Sc-2.The pupal of Xestia c-nigrum was insensitive to the seven strains of the entomopathogenic nematode,and the eclosion of Xestia c-nigrum was promoted by the entomopathogenic nematode.Juvenile 3 was considered as the optimum control stage of Xestia c-nigrum to entomopathogenic nematode.

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Last Update: 2013-02-20