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Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2013年01期
Page:
38-45
Research Field:
Publishing date:

Info

Title:
Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment
Author(s):
GAI Jiang-nan; ZHANG Bin-bin; WU Yao; LI Wen-bin
Key Laboratory of Soybean Biology in Chinese Ministry of Education,Key Laboratory of Biology and Breeding/ Genetics of Chinese Agriculture Ministry,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
Keywords:
Soybean; Adventitious embryos suspension system; Particle bombardment; Genotype screening
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2013.01.009
Abstract:
In this work,we used the immature cotyledons of 32 soybean genotypes to induce adventitious embryos and compared the percentage of primary embryo induction,percentage of secondary embryo induction and induction efficiency after 30 days inducing culture.The results showed that L155,Kenfeng 16,Suinong 25 and Jiyu 91 had higher frequency of adventitious embryos induction.Furthermore,L155 was selected to further establish the soybean suspension culture system.The secondary embryogenesis systems were used for GUS gene transformation by particle gun bombardment method.The GUS staining results showed that the GUS gene was imported into soybean adventitious embryos cells and successfully expressed.

References:

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Last Update: 2013-02-20