|Table of Contents|

Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2013年01期
Page:
33-42
Research Field:
Publishing date:

Info

Title:
Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”
Author(s):
ZHANG Li-wei 1 FAN Ying-lun 23 NIU Teng-fei? 1 ZHANG Wen-hui1 ZHAO Yu-jie1 LIU Li-ke 13
1.School of Life Science,Liaocheng University;
2.School of Agricultural Science,Liaocheng University;
3.Institute of Crop Breeding,Liaocheng University,Liaocheng 252059,Shandong,China
Keywords:
SoybeanPhysical and chemical mutagenesisJihuang13
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2013.01.008
Abstract:
Mutants and mutant population are the foundation for gene function analysis and crop genetic improvement.Seed of soybean cultivar “Jihuang 13” were treated with ethyl methane sulfonate(EMS),sodium azide(NaN3)and nitrogen ion beam implantation respectively for screening mutants and constructingmutant population.Fifteen stem related mutants,19 leaf related mutants,31 floral related mutants,3 seed related mutants,and 18 maturity related mutants were obtained after selected in M2generation,and validated in M3and M4 generation.Because there were 9 mutants possessing composite phenotypes,77 genetically stable mutants were finally screened out.Further genetic analyses for a male sterile mutant and a black seed color mutant were made in F2 segregating population respectively.The results showed that the phenotypes of these two mutants were both controlled by a single recessive gene.The mutants and mutant population obtained would lay a foundation for functional genomics analyses and genetic improvement of soybean.

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Last Update: 2013-02-20