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Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2013年01期
Page:
23-32
Research Field:
Publishing date:

Info

Title:
Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean
Author(s):
LU Qing-yao;  ZHAO Lin;  LI Dong-mei;  GU Yue-jiao;  ZHANG Yan-wei;  LV Qing-xue;  LIU Li-xue;  LI Wen-bin
Key Laboratory of Soybean Biology in Chinese Ministry of Education,Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry, Northeast Agricultural University,Harbin 150030,Heilongjiang,China
Keywords:
Soybean;  GmRAV;  Shoot regeneration; Cytokinins
PACS:
Q954.48,S565.1
DOI:
10.3969/j.issn.1000-9841.2013.01.006
Abstract:
RAV is a member of the ERF/AP2 transcription factor family.We use explants of Arabidopsis rav T-DNA insertion homozygous mutants and inhibition(-i)of GmRAV in soybean as experimental material to carry out callus induction.Various concentration of exogenous Cytokinins 2-ip and 6-BA were added in the shoot-inducing medium and the changes in the average number of shoots and the shoot regeneration rates were investigated.It turned out that Cytokinins had positive effects on the shoot regeneration,in addition,the average number of shoots and shoot regeneration rates of Arabidopsis rav mutants and GmRAV-i soybean plants would decrease by 2-ip and 6-BA.It proved that the function of RAV homologs was conserved and RAV promoted shoot regeneration in the presence of Cytokinins.

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Last Update: 2013-02-20