|Table of Contents|

Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2013年01期
Page:
19-27
Research Field:
Publishing date:

Info

Title:
Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers
Author(s):
ZHANG Chun-bao1;  LI Yu-qiu1;  PENG Bao1;  WANG Peng-nian2;  DONG Ying-shan1;  ZHAO Li-mei1
1.Soybean Research Institute,Jilin Academy of Agricultural Sciences,Changchun 130033；
2.College of Agronomy,Jilin Agricultural University,Changchun 130022,Jilin,China
Keywords:
Soybean; Sterile line; Maintainer line; Hybrid; Molecular markers
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2013.01.005
Abstract:
By extracting the mitochondrial DNA of cytoplasmic male sterile line and its maintainer line,screening with 100 ISSR primers,and it was found that the amplification product of primer ISSR829 had differences in sterile line and its maintainer.Then sequenced the different fragments and found that the sequence was only exist in the maintainer mitochondrial genome,and upstream of mitochondrial gene Atp6 by 2 000 bp.It was suggested that the sequence has a relationship with infertility.Designed primers according to the sequence and detected female parent and its maintainer line of five soybean hybrids,and found that sterile line did not appear specific fragment,but a target fragment corresponding maintainer lines.It was confirmed that the specific fragments was converted to a SCAR marker,which could be used for testing the seed purity of cytoplasmic male sterile line.

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Last Update: 2013-02-20