|Table of Contents|

Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2013年01期
Page:
12-18
Research Field:
Publishing date:

Info

Title:
Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)
Author(s):
WANG Ming-xia; CUI Xiao-xia; XUE Chen-chen; XU Jin-yan; XUE Dong; GUO Na; XING Han
National Center for Soybean Improvement,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China
Keywords:
Gateway technology; Soybean; RNA interference; GmHAL3a gene
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2013.01.004
Abstract:
Halotolerance 3 gene was isolated from soybean(Glycine max)by homology cloning,designed as GmHAL3a.Tissue specific analysis found that GmHAL3a expressed mainly in root,relatively lower in pod and lowest in leaf. RT-PCR revealed that the expression of GmHAL3a was induced by salt,LiCl and sorbitol.Bioinformatics analysis indicated that -GmHAL3a had two transmembrane helices regions and two conserved domains,flavin mononucleotide(FMN)binding sites and 4’-phosphopantothenoylcysteine(PPC)decarboxylase activity sites,and was possibly localized in chloroplast.We cloned two segments of GmHAL3a,ligased into entry clone vector(pDONR221)by BP reaction,and then ligased into RNAi transformation vector pB7GWIWG2(Ⅱ)by LR reaction.Finally we transformed the two vectors into Agrobacterium tumefaciens EHA105.The results provide foundation for further research the function of GmHAL3a? gene in soybean.

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Last Update: 2013-02-20