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Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2013年01期
Page:
8-17
Research Field:
Publishing date:

Info

Title:
Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max
Author(s):
LI Cai-yun1; 2;  YU Yong-liang2;  YANG Hong-qi2;  DONG Wei2;  TIAN Bao-ming1;  LIANG Hui-zhen2
1.Department of Bioengineering,Zhengzhou University,Zhengzhou 450001,Henan;
2.Sesame Research Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China
Keywords:
Glycine max; Lipid-transfer proteins; Abiotic stress; Expression analysis
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2013.01.003
Abstract:
In this study,a gene which have 94% identity with the SiLTP3 was cloned and named as GmLTP3. GmLTP3 encodes a lipid-transfer protein(LTP)that have 99% identity with the known Glycine max lipid transfer protein.The transcriptional level of GmLTP3 was quantitatively determined using realtime-PCR,it was expressed in almost all the tissues,such as root,stem,leave,flower and seed.It was highly expressed in alabastrum,7-days old stem and 3-days germinating seed,while it was lowly expressed in root and leave.When treated by abiotic stress,such as IAA,ABA,PEG and NaCl,the expression level was down-regulated after treated 2 hours,but was up-regulated after treated 12 hours,while it was downregulated consistently after treated by cold stress.

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Last Update: 2013-02-20