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Construction of the RNAi Expression Vector of Soybean 7S Protein β-subunit Gene(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年06期
Page:
1021-1023
Research Field:
Publishing date:

Info

Title:
Construction of the RNAi Expression Vector of Soybean 7S Protein β-subunit Gene
Author(s):
DAI Jia-le; MA Jian; FU Yong-ping; QU Jing; WANG Pi-wu
Agronomy College,Jilin Agricultural University,Changchun 130118,Jilin,China
Keywords:
RNA interference; 7S protein β-subunit; Expression vector
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.06.035
Abstract:
In this study we used β-subunit gene of 7S protein as the target genes(Gene number:AB030840),then obtained 398 bp core sequence of β-subunit gene by RT-PCR and constructed RNAi expression safe plant vector BAR-7αp-β，with a herbicide resistant BAR gene as selection marker.The result of PCR,molecular biology detection demonstrated that gene expression vector of 7S protein β-subunit was successfully constructed.This study provided foundation for the application of RNA interference technology to reduce soybean allergens and improve quality of soybean proteins.

References:

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