Optimization on Production Parameters of Rapid Dissolving Soybean Protein Isolate(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2012年06期

Page:

996-1001

Research Field:

Publishing date:

Info

Title:

Optimization on Production Parameters of Rapid Dissolving Soybean Protein Isolate

Author(s):

YAN Han¹; NIE Jian-qi²; GE Pei-fu¹; HUAN Xu-ying¹

1.College of Science，Liaoning Technical University，Fuxin 123000，Liaoning；

2.School of Biotechnology,Jiangnan University，Wuxi 214122,Jiangsu,China

Keywords:

Soybean protein isolate; Enzymatic hydrolysis; Solubility; Instant solubility

PACS:

TS201.2

DOI:

10.3969/j.issn.1000-9841.2012.06.030

Abstract:

In this study,in order to increase instant solubility of soybean protein isolate(SPI),neutral protease and papain were used as tool enzyme,enzymatic hydrolysis technology was optimized by single factor experiment and response surface optimization design,with hydrolysis degree as the index.Then blended with phospholipids and cane sugar to further improve the solubility.The optimum conditions of enzymatic hydrolysis were at pH7.08 and 51.6℃ for 3 h with the enzyme concentration of 22.45 mg·g^-1 substrate.Under this enzymatic hydrolysis conditions,the hydrolysis degree and solubility of SPI reached 20.3% and 72%,respectively.After blended with 0.8% phospholipids and 3.0% cane sugar,the final solubility of SPI reached 92.9%,and the SPI sample could quickly dissolved into water without aggregation.

References:

[1]田琨,管娟,邵正中,等.大豆分离蛋白结构与性能[J].化学进展,2008,20(4):570.(Tian K,Guan J,Shao Z Z,et al.Structural and functional study of soybean protein isolation[J].Progress in Chemistry,2008,20(4):570.)

[2]肇立春.大豆分离蛋白生产工艺探讨[J].粮油加工与食品机械,2006（1）:50-51.(Zhao L C.Discussion on the production technology of soybean protein isolate[J]. Machinery For Cereals Oil and Food Processing,2006（1）:50-51.)

[3]华欲飞.大豆分离蛋白性能优化关键技术[J].中国油脂,2001,26(6):79-81.(Hua Y F.Critical technology in the optimization of soy protein isolate performance[J].China Oils and Fats,2001,26(6):79-81.)

[4]赵敏生.速溶性大豆分离蛋白的生产方法[J].食品科技,2002(8):17.(Zhao M S.Instantly soy protein isolate production methods[J].Food Science and Technology,2002(8):17.)

[5]Molina E,Papadopoulou A,Ledward D A.Emulsifying properties of high pressure treated soy protein isolate and 7S and 11S globulins[J].Food Drocolloids,2001,15:263-269.)

[6]田其英,华欲飞.大豆蛋白溶解性研究[J].粮食与油脂,2006（6）:8.(Tian Q Y,Hua Y F.Study on the solubility of soybean protein[J].Cereals and Oils,2006（6）:8.)

[7]张华山.酶解大豆分离蛋白的制备及应用[J].适用技术市场,1999(7):20-21.(Zhang H S.Preparation and application of enzymatic soybean protein isolate[J].Applicable Technology Market,1999(7):20-21.)

[8]迟晓星,张涛,张园园.酶法水解大豆分离蛋白的研究[J].中国食物与营养,2007(1):33-35.(Chi X X,Zhang T,Zhang Y Y.Study of enzymatic hydrolysis of soybean protein isolate[J].Food and Nutrition in China,2007(1):33-35.)

[9]刘进杰,张玉香,冯志彬,等.超声和Alcalase酶复合处理水解大豆分离蛋白工艺研究[J].食品科学,2010,31(14):84-87.(Zhang J J,Zhang Y X,Feng Z B,et al.Ultrasonic-assisted alcalase hydrolysis of soybean protein isolate[J].Food Science,2010,31(14):84-87.)

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