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Detection of Transgenic Soybean and Its Food by MPCR-DHPLC Method(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年06期
Page:
976-979
Research Field:
Publishing date:

Info

Title:
Detection of Transgenic Soybean and Its Food by MPCR-DHPLC Method
Author(s):
TAO Ran; GUO Shun-tang
College of Food Science & Nutritional Engineering,China Agricultural University,Beijing 100083
Keywords:
Multiplex PCR; Denaturing high-performance liquid chromatography(DHPLC); Genetically modified soybean
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.06.025
Abstract:
The aim of this work was to develop a method for simultaneous detection of a variety of genetically modified(GM)soybean ingredients in foods and to identify the lines of transgenic soybean by using multiplex polymerase chain reaction(PCR)coupled with denaturing high performance liquid chromatography(DHPLC)assay.Results showed that the multiplex PCR-DHPLC method was highly specific for the target genes with 0.078 ng·μL-1 of high sensitivity.Detection sensitivity of standard plasmid was 1×103 copy·μL-1.Identification and detection of genetically modified soybean ingredients in GT-40-3-2,Mon89788,A5704-12,cookies,seasoning powder containing soybean,dried tofu and non-genetically modified black soybean were effective.Multiplex PCR-DHPLC method developed in this work will be a better alternative for the rapid and high-throughput detection of many genetic modification events in soybean and its processed food simultaneously.

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Last Update: 2014-08-20