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Title:: Effects of Nitrogen Application on Growth and Nitrogen Fixation in Nodulation and Non-nodulation Soybean Isolines

Author(s):: SHI Hai¹; 2; MIAO Shu-jie¹; LIU Ju-dong¹; ZHOU Ke-qin¹; 1.Key Laboratory of Mollisols Agroecology,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150081,Heilongjiang;
2.Graduate University of the Chinese Academy of Sciences,Beijing 100049,China

Keywords:: Carbon and nitrogen metabolism; Nodulation; Nitrogen fixation; Isoline soybean; Threshold

PACS:: S565.1

DOI:: 10.3969/j.issn.1000-9841.2012.06.022

Abstract:: To illustrate the influence of nitrogen fertilizer application on the growth and nitrogen fixation in soybean,the study on the effect of the different N application levels on growth,carbon and nitrogen metabolism,nodulation and nitrogen fixation in soybean under sand culture condition,was carried out with nodulation and non-nodulation soybean isolines.The results showed that N application levels directly impacted soybean biomass.The biomass of nodulation soybean reached the maximum value of 9.16 g·plant^-1 at N₂ level(54 mg urea·kg^-1 sand),while the non-nodulation soybean reached the maximum of 6.36 g·plant^-1 at N₄ level(216 mg urea·kg^-1 sand).There was similar trend of carbon and nitrogen metabolism affected by N level.In addition,nodule number reached the largest number(205 per plant)at N₂ level,and there were significant differences between N₂ and other treatment,exception of N₄ level(P<0.01).The amount of symbiotic nitrogen fixation reached the maximum of 275.92 mg·plant^-1 at N₂ level,and there were significant differences between N₂ and N₃(N₄ or N₅)(P<0.01).These results showed that there was obvious difference between nodulation and non-nodulation soybean isoline to the demand of exogenous nitrogen.To take full advantage of symbiotic nitrogen fixation of soybean,nitrogen repression threshold was N₂ level(54 mg·kg^-1 sand)under this experiment conditions.

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Effects of Nitrogen Application on Growth and Nitrogen Fixation in Nodulation and Non-nodulation Soybean Isolines(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

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