|Table of Contents|

Study on the Role of NH4+-N and NO3--N on Soybean Nutrition Using Labeled 15N(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年06期
Page:
911-914
Research Field:
Publishing date:

Info

Title:
Study on the Role of NH4+-N and NO3--N on Soybean Nutrition Using Labeled 15N
Author(s):
DONG Shou-kunLIU Li-junMA Chun-meiSUN Cong-shuGONG Zhen-pingZU Wei
Agronomy College,Northeast Agricultural University,Northeast Area Crop Cultivation Science Observation Experiment Station of Ministry of Agriculture,Harbin 150030,Heilongjiang,China
Keywords:
SoybeanNitrogenNH4+-NNO3--N
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.06.011
Abstract:
This paper studied the role of NH4+-N and NO3--N on soybean(Dongnong47)nutrition using 15N trace technique and tissue culture.The results showed that soybean appeared ammonium salt toxicity when NH4+-N was used as nitrogen source under aseptic condition,soybean could grow normaly when NO3--N and mixed nitrogen (NO3--N∶NH4+-N=1∶1) were used as nitrogen source.The total biomass reached maximum when mixed nitrogen were used as nitrogen source,NO3--N took second place,NH4+-N reached minimum.Biomass differences between the different forms of nitrogen reached a significant level.Exogenous nitrogen absorbed by soybean plants increased with nitrogen level.When the ratio of NH4+-N and NO3--N in culture medium was 1∶1,the ratio of NH4+-N and NO3--N absorbed by soybean was approximately 1.5∶1,and the ratios of NH4+-N and NO3--N absorbed by leaf,stem and root were 1.6∶1,1.4∶1 and 1.6∶1,respectively.Results suggest nitrate nitrogen could eliminate ammonium salt toxicity and promoted nitrogen absorption and utilization in soybean.

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Last Update: 2014-08-19