Effect of IBA and 6-BA Ratio on Adventitious Shoot Induction from Hypocotyl in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2012年05期

Page:

725-730

Research Field:

Publishing date:

Info

Title:

Effect of IBA and 6-BA Ratio on Adventitious Shoot Induction from Hypocotyl in Soybean

Author(s):

GUO Qiu-yun¹; 2; WANG Ping¹; 2; LIU Zhao-pu²

1.School of Marine Science and Technology,Huaihai Institute of Technology,Lianyungang 222005,Jiangsu;

2.Key Laboratory of Marine Biology of Jiangsu Province,College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China

Keywords:

Soybean; Hypocotyl; Adventitious shoot; IBA; 6-BA

PACS:

S565.1

DOI:

10.3969/j.issn.1000-9841.2012.05.007

Abstract:

Hypocotyl from soybean Heinong 44 was used as the explants to investigate the effects of IBA and 6-BA and the concentration ratio on the adventitious shoot induction and elongation.The higher induction rate of adventitious shoots from hypocotyls was observed in the induction MS medium without 6-BA while the seeds germinated on MS medium+1.0 mg·L^-16-BA.Induction rate and number of adventitious shoots reached 96.69% and 2.31(shoot length≥0.5 cm)respectively cultured for 14 days on induction MS medium with addition of 0.1 mg?L-1IBA.Shoot induction medium with higher concentration of 6-BA and IBA significantly reduced the induction and elongation of adventitious shoots from hypocotyl of soybean.

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