|Table of Contents|

Analysis of Gene Expression Underlying Soybean Isoflavone Synthesis Relative Enzymes at Different Growth Stages(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年06期
Page:
887-893
Research Field:
Publishing date:

Info

Title:
Analysis of Gene Expression Underlying Soybean Isoflavone Synthesis Relative Enzymes at Different Growth Stages
Author(s):
WANG Yan; WU Lin; SUN Meng-yang; HAN Ying-peng; ZHAO Xue; TENG Wei-li; LI Wen-bin
Key Laboratory of Soybean Biology in Chinese Ministry of Education,Key Laboratory of Biology and Breeding/Genetics of Chinese Agriculture Minstry,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
Keywords:
Soybean isoflavone; Isoflavone synthesis relative enzyme genes; Real-time PCR; Relative expression quantity
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.06.006
Abstract:
Real-time PCR was used to measure the relative expression quantity of seven isoflavone synthetic enzyme related genes(PAL,C4H,4CL,CHS,,IFCHIS and F3H)in high isoflavonecv.Zhongdou27,low isoflavone cv. Jiunong20,as well as their derived 130 F5∶11 RILs.High performance liquid chromatography(HPLC)was adopted to survey isoflavone content in pod at different growth stages,at the same time,correlations between total/individual isoflavone content and the expression of partial genes in F5∶11 RILs were performed using SAS 8.2.The relative expression quantity of（PAL，CHI，IFS and F3H was generally increased in reproductive stage than vegetative stage.Relative expression quantity of PAL,C4H,CHS and IFS gene in leaf appeared a peak value at R1 stage,and the expression quantity of most selected genes appeared significant difference in pod between two variety was at R6 stage.At R6 stage,the relative expression quantity of PAL gene manifested positive correlation with genistein(GT)and total isoflavone content(TI);CHS exhibited positive relevance with daidzein(DZ),GT and TI but negative with glycitein(GC);IFS displayed positive correlation with DZ;F3H showed negative correlation with individual and total isoflavone contents.Results will faciliate the understanding of genetic mechanism of the variation underlying isoflavone content.

References:

[1]Dhaubhadel S,McGarvey B D,Williams R,et al.Isoflavonoid biosynthesis and accumulation in developing soybean seeds[J].Plant Molecular Biology,2003,53:733-743.

[2]Brouns F.Soya isoflavones:a new and promising ingredient for the health food sector[J].Food Research International,2002,35:187-193.

[3]Murphy S E,Lee E A,Woodrow L,et al.Genotype ×environment interaction and stability for isoflavone content in soybean[J].Crop Science,2009,49:1313-1321.

[4]Yu O,Shi J,Hession A O,et al.Metabolic engineering to increase isoflavone biosynthesis in soybean seed[J].Phytochemistry,2003,63:753-763.

[5]Gutierrez-Gonzalez J J,Wu X L,Gillman J D,et al.Intricate environment-modulated genetic networks control isoflavone accumulation in soybean seeds[J].BMC Plant Biology,2010,10:105.

[6]易金鑫，徐照龙，王峻峰，等.GmCHS8和GmIFS2基因共同决定大豆中异黄酮的积累[J].作物学报,2011,37(4):571-578.(Yi J X,Xu Z L,Wang J F,et al.Accumulation of soybean isoflavone determined by?GmCHS8?and GmIFS2?together[J].Acta Agronomica Sinica,2011,37(4):571-578.)

[7]董李平.大豆异黄酮代谢机理的研究[D].重庆：西南农业大学,2003:26-47.(Dong L P.Study of soybean isoflavone metabolic mechanism[D].Chongqing:Southwest Agricultural University,2003:26-47.)

[8]Kim J A,Chung I M.Change in isoflavone concentration of soybean (Glycine maxL.)seeds at different growth stages[J].Journal of the Science of Food and Agriculture,2007,87(3):496-503.

[9]张大勇.大豆异黄酮含量影响因素的分析[D].沈阳：沈阳农业大学，2009：80-94.(Zhang D Y.Analysis of factors effecting soybean isoflavone content[D].Shenyang:Shenyang Agricultural University,2009:80-94.)

[10]Wall S J,Edward D R.Quantitative reverse transcription_polymerase chain reaction (TR_PCR):A comparison of prime_dropping,competitive,and real-time RT-PCR[J].Analytical Biochemistry,2002,300(2):269-273.

[11]Fehr W R,Caviness C E.Stages of soybean development[R].Iowa:Iowa State University,1977:1-11.

[12]Ritchie S W,Hanway J J,Thompson H E,et al.How a soybean plant develops[R].Iowa:Iowa State University,1996.

[13]Zeng G L,Li D M,Han Y P,et al.Identification of QTL underlying isoflavone contents in soybean seeds among multiple environments[J].Theoretical and Applied Genetics,2009,118:1455-1463.

[14]Livak K J,Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT?method[J].Methods,2001,25(4):402-408.

[15]石海燕，张玉星.木质素生物合成途径中关键酶基因的分子特征[J].中国农学通报，2011,27（5）：288-291.(Shi H Y,Zhang Y X.Molecular characterization of key enzyme genes related to the pathway of lignin biosynthesis[J].Chinese Agricultural Science Bulletin,2011,27(5):288-291.)

[16]Yin Z,Meng F,Song H,et al.Expression quantitative trait loci analysis of two genes encoding rubisco activase in soybean[J].Journal of Plant Physiology,2010,152(3):16-25.

[17]秦胜华，颜秀娟，李明姝，等.大豆源库流关系研究浅析[J].湖南农业科学,2010，17：54-56.(Qin S H,Yan X J,Li M S,et al.Analysis of relationships among source,sink and flux of soybean[J].Hunan Agricultural Sciences,2010,17:54-56)

[18]闺兴中,贾观钦.大豆鼓粒期源库关系研究[J].江苏农业学报,1991,7(4):13-19.(Gui X Z,Jia G Q.Analysis of relationships among source and sink at soybean seed-filling period[J].Jiangsu Journal of Agricultural Science,1991,7(4):13-19.)

[19]王玲玲,杜吉到,郑殿峰,等.大豆源库流关系的研究进展[J].大豆科学，2009,28(1):167-171.(Wang L L,Du J D,Zheng D F,et al.Advances in the studies of relation among source sink and flux of soybean[J].Soybean Science,2009,28(1):167-171.)

[20]刘洪梅,李英,卜贵军,等.大豆豆荚光合物质转运与分配对籽粒发育的影响[J].核农学报，2008,22（4）：519-523.(Liu H M,Li Y,Bu G J，et al.Effect of photosynthetic material transporting and distributing of soybean pod to seed development[J].Journal of Nuclear Agricultural Sciences ,2008,22(4):519-523.)

[21]柳青，李欣达，王玉民，等.RNAi干扰的F3H基因转化大豆的研究[J].北京农业，2010(15):7-11.(Liu Q,Li X D,Wang Y M,et al.Studies on transformation of F3H?gene interfered by RNAi in soybean[J].Beijing Agriculture,2010(15):7-11.)

Memo

Memo:
-
Last Update: 2014-08-19