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Cloning of AtGPAT9 cDNA from Arabidopsis and Its Transformation of Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年06期
Page:
878-881,886
Research Field:
Publishing date:

Info

Title:
Cloning of AtGPAT9 cDNA from Arabidopsis and Its Transformation of Soybean
Author(s):
WU Xing; ZHAO Huan-huan; ZHANG Feng; LI Hong-wei; WANG Mao-yan
College of Life Sciences,Inner Mongolia Agricultural University,Hohhot 010018,Inner Mongolia,China
Keywords:
Glycerol-3-phosphate acyltransferases(GPATs); Gene cloning; Transformation of soybean
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.06.004
Abstract:
Glycerol-3-phosphate acyltransferases(GPATs)play crucial role in the biosynthesis of triacylglycerols(TAGs)which are major form of the storage oils accumulated in developing seeds,and the enzymes catalyze the acylation of glycerol-3-phosphate at the sn-1 position and result in the formation of lysophosphatidic acid,which is the initial step of TAG biosynthesis.The endoplasmic reticulum-expressed AtGPAT9,a member of the GPAT family in Arabidopsis thaliana,was predicted to play an important role in the reaction.In the present study,the complete coding region cDNA of AtGPAT9 was cloned by using reverse transcriptase-mediated PCR,and the gene’s plant expression vector was constructed.Then soybean transformation was performed by the Agrobacterium tumefaciens-mediated cotyledon node method,and the regenerated plants resistant to selective agent phosphinothricin/PPT were obtained.

References:

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Last Update: 2014-08-19