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Prokaryotic Expression and Protein Purification of Soybean GmGBP1 Gene(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年06期
Page:
874-877
Research Field:
Publishing date:

Info

Title:
Prokaryotic Expression and Protein Purification of Soybean GmGBP1 Gene
Author(s):
ZHANG Yan-weiLIU Li-xueZHAO LinGU Yue-jiaoLU Qing-yaoSONG Xian-pingLI Wen-bin
Key Laboratory of Soybean Biology in Chinese Ministry of Education,Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
Keywords:
SoybeanSKIPProkaryotic expressionInclusion bodyPurification
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.06.003
Abstract:
A new SKIP homolog in soybean was constructed into prokaryotic expression vector pGEX-6p-1 and then transformed into E.coli BL21(DE3)to get the expression protein for further study.The result indicated that an 95 kDa recombinant protein was expressed with the treatment of 0.1 mmol.L-1IPTG for 8 h at 37℃,the recombinant protein was confirmed to mainly existed in inclusion body form by SDSPAGE.The high uality recombinant protein was obtained through GST column purification after the inclusion body was dissolved with 8 mol.L-1 urea.

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Last Update: 2014-08-07