Quantitative Detection of Genetically Modified Soybean BPS-CV127-9(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2012年05期

Page:

808-812

Research Field:

Publishing date:

Info

Title:

Quantitative Detection of Genetically Modified Soybean BPS-CV127-9

Author(s):

LI Ning¹; 2; ZHAO Lei³; SUN Hong-wei¹; LI Fan¹; YANG Shu-ke¹; LU Xing-bo¹

1.Institute of Plant Protection,Shandong Academy of Agricultural Sciences,Jinan 250100,Shandong;

2.School of Public Health,Jining Medical University,Jining 272067,Shandong;

3.College of Life Science,Shandong Normal University,Jinan 250014,Shandong,China

Keywords:

Event-special; Real-time PCR; Genetically modified soybean

PACS:

S565.1

DOI:

10.3969/j.issn.1000-9841.2012.05.026

Abstract:

A quantitative method to detect the transgenic soybean BPS-CV-127-9 by using real-time PCR technique based on fluorescence dye SYBR Green I was investigated in this study.The endogenous lectin gene and the 5′ flanking sequences of BPS-CV-127-9 were amplified through the specific primers,and the transgenic content was then calculated according to the standard curve equation.Meanwhile the specificity of PCR amplification was analyzed by corresponding melting curves.The results showed that the standard curves of lectin and the 5′ flanking sequences of BPS-CV-127-9 genes have good linear relationship,and their R2 values were 0.999 and 0.998,respectively.The coefficient of variance was 1.50%-18.51% and standard deviation was 0.02-0.07.Four mixed samples with genetically modified contents of BPS-CV-127-9 was 0.05%,0.1%,0.5% and 1% respectively were detected,and the detection results agreed well with actual value.In conclusion,this method was fast,sensitive,simple,accurate,specific and of high throughput,and could be used to detect transgenic soybean BPS-CV-127-9 quantificationally.

References:

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