|Table of Contents|

Optimization on Cultivation Conditions of High Effective Chlorimuron-Ethyl Degrading Strain(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年05期
Page:
804-807
Research Field:
Publishing date:

Info

Title:
Optimization on Cultivation Conditions of High Effective Chlorimuron-Ethyl Degrading Strain
Author(s):
YAN Hong; WEI Yan-yang
College of Chemical and Environmental Engineering，Harbin University of Science and Technology，Key Laboratory of Green Chemical Technology of College of Heilongjiang Province，Harbin 150040，Heilongjiang，China
Keywords:
Chlorimuron-ethyl; Chlorimuron-ethyl degrading strain; Cultivation conditions; Optimization
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.05.025
Abstract:
The high effective Chlorimuron-ethyl degrading strains(A2,A5 and A6),which used in this study,were isolated and selected in early experiments.The effects of carbon sources,nitrogen source,pH,temperature,and chlorimuron-ethyl concentration on the growth were explored in this experiment.The results showed that the best carbon source and nitrogen source were lactose and peptone,respectively,for these three strains.As for A2,optimal concentration of lactose and peptone was 20 g·L-1 and 10 g·L-1,respectively.Suitable pH range of growth for A2 was 5.4-7.0,whereas the optimal pH was 6.0.Suitable temperature range of growth was 25-36℃,whereas the optimum temperature was 28℃.The suitable concentration of lactose and peptone for A5 and A6 were 15 g·L-1 and 10 g·L-1,respectively.Appropriate pH range was 5.4-8.0,and the optimal pH was 5.4.Suitable temperature range of growth for A5 and A6 was 25-36℃,and the optimum temperature was 28℃.The biggest bacterium density(OD600)of the three strains(A2,A5,and A6)could reach to 1.802,1.804 and 1.887,respectively,which were 15.5,8.4 and 42.9 times higher than wild strains,under the optimum culture condition for 60 h.

References:

[1]马艳军,程茁,乔琦,等.氯嘧磺隆对大豆根圈土壤3种酶活性的影响[J].现代农业科学,2009,16(4):1-3.（Ma Y J,Cheng Z,Qiao Q,et al.Effect of chlorimuron-ethyl on three soil enzyme activity in soybean rhizosphere[J].Modern Agricultural Science,2009,16(4):1-3,20.）

[2]陶波,何钟佩.作保灵（TNA）对大豆保护效应及机制的研究[J].大豆科学,2000,19(2):189-192.（Tao B,He Z P.Protective action and mechanism of TNA on soybean[J].Soybean Science,2000,19(2):189-192.）

[3]李丽,谢明,周淑云,等.氯嘧磺隆高效降解细菌的分离与筛选[J].中国生物防治学报,2009,25(1):70-73.（Li L,Xie M,Zhou S Y,et al.Isolation and screening of chlorimuron-ethyl degrading becteria from soil[J].Chinese Journal of Biological Control,2009,25(1):70-73.）

[4]朱连丹.土壤残留氯嘧磺隆降解菌的筛选[D].长春:吉林农业大学,2009:11.（Zhu L D.Screening of chlorimuron-ethyl degrading fungi in soil[D].Changchun:Jilin Agricultural University,2009:11.）

[5]张希国,张伟,李德斌.氯嘧磺隆降解菌培养条件的优化[J].林业科技情报,2011,43(2):10-12.（Zhang X G,Zhang W,Li D B.Optimization of cultivation conditions of chlorimuron-ethyl degrading bacteria[J].Forestry Science and Technology Information,2011,43(2):10-12.）

[6]欧红香,闫永胜,毛艳丽,等.1株高效微生物絮凝剂产生菌的筛选及培养条件优化[J].安徽农业科学,2009,37(31):15139-15140,15168.（Ou H X,Yan Y S,Mao Y L,et al.Screening of an efficient producing bacterium of microbial flocculant and optimization on its culture condition[J].Journal of Anhui Agricultural Sciences,2009,37(31):15139-15140,15168.）

[7]滕春红.氯嘧磺隆对土壤微生态的影响极其高效降解真菌的研究[D].哈尔滨:东北农业大学,2006:35.（Teng C H.Effects of chlorimuron-ethyl on the soil microecosystem and research of chlorimuron-ethyl degrading fungi[D].Harbin:Northeast Agricultural University,2006:35.）


Memo

Memo:
-
Last Update: 2014-08-19