Cloning and Expression Analysis of Regeneration Related Gene GmESR1 in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2012年05期

Page:

731-733,738

Research Field:

Publishing date:

Info

Title:

Cloning and Expression Analysis of Regeneration Related Gene GmESR1 in Soybean

Author(s):

LIU Ming¹; SU An-yu²; LI Jing¹; SUN Jing¹; WANG Min¹; LI Si-nan¹; LI Wen-bin¹; WU Xiao-xia¹

1.College of Agronomy,Northeast Agricultural University,Key Laboratory of Soybean Biology in Chinese Ministry of Education,Harbin 150030,Heilongjiang;

2.School of Resource & Environment,Northeast Agricultural University,Harbin 150030,Heilongjiang,China

Keywords:

Soybean; Regeneration; GmESR1

PACS:

S565.1

DOI:

10.3969/j.issn.1000-9841.2012.05.008

Abstract:

In order to establish an efficient and stable soybean regeneration system,a soybean regeneration-related gene GmESR1 was cloned through candidate gene homology-based method and analyzed its function.Our results showed that the complete length and coding region of GmESR1 was 1 164 bp,981 bp respectively,and encoded a peptides consisting of 326 amino acids.Protein sequence analysis indicated that GmESR1 contains an AP2/ERF domain,and belongs to the AP2 family,which contained members of cytokinin inducible transcription factors that were extensively involved in plant resistance against stress and transcriptional regulation during shoot regeneration.Blast search showed that GmESR1shared high homology with Arabidopsis ESR1 gene,which was also a member of the AP2 family.Exogenous hormone treatment experiment showed that the expression level of GmESR1 was increased under the cytokinin treatment.

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