Isolation and Expression Pattern Analysis of LRR Kinase Gene Response to Osmotic Stress from Glycine soja (PDF)

¡¶´ó¶¹¿Æѧ¡·[ISSN:1000-9841/CN:23-1227/S]

Issue:

2012Äê05ÆÚ

Page:

718-724

Research Field:

Publishing date:

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Title:

Isolation and Expression Pattern Analysis of LRR Kinase Gene Response to Osmotic Stress from Glycine soja

Author(s):

YANG Liang¹; LI Xiang-yu²; GAO Peng³; CHEN Qing-yuan⁴; GUO Yu-shuang⁴

1.Key Laboratory of Plant Virology of Fujian Province,Institute of Plant Virology,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian;

2.Crop Breeding Instituce,Heilongjiang Academy of Agricultural Science,Harbin 150086,Heilongjiang;

3.College of Horticulture,Northeast Agricultural University,Harbin 150030,Heilongjiang;

4.Guizhou Institute of Tobacco Science,Guiyang 550083,Guizhou,China

Keywords:

Glycine soja; LRR-RLK; Osmotic stress

PACS:

S565.1

DOI:

10.3969/j.issn.1000-9841.2012.05.006

Abstract:

One EST induced by cold represents a leucine-rich repeat receptor-like kinase(LRR-RLK)gene was identified according to the gene expression profiles of Glycine soja leaves under osmotic stresses previously constructed in our laboratory.The full-length cDNA was successfully retrieved by RACE,which was 2 264 bp in length and contains a complete ORF encoding 714 amino acids.The bioinformatics analysis showed that,the GsLRPK protein had an N-terminal signal sequence(residues 1 to 22),extracellular leucine-rich repeats,a single transmembrane region,and a cytoplasmic catalytic domain,considered crucial for kinase activity.What¡¯s more,semi-quantity PCR result showed that the GsLRPK was regulated by different stresses treatment,such as drought,ABA,4¡æ cold and high salinity,and could function in the signaling of osmotic stresses.

References:

[1]Yang L,Ji W,Gao P,et al.GsAPK an ABA-activated and calcium-independent SnRK2-type kinase from G.soja mediates the regulation of plant tolerance to salinity and ABA stress[J].PLoS ONE,2012,7(3):e33838.doi:10.1371/journal.pone.0033838.

[2]Zhou Q Y£¬Tian A G£¬Zou H F,et al.Soybean WRKY-type transcription factor genes,GmWRKY13,GmWRKY21 and GmWRKY54,confer differential tolerance to abiotic stresses in transgenic Arabidopsis?plants[J].Plant Biotechnology Journal,2008,6(5):486-503.

[3]Yang L,Ji W,Zhu Y M,et al.GsCBRLK,a calcium/calmodulin-binding receptor-like kinase,is a positive regulator of plant tolerance to salt and ABA stress[J].Journal of Experimental Botany,2010,61(9):2519-2533.

[4]Yoshioka K,Shinozaki K.Signal crosstalk in plant stress responses[M].Wiley-Blackwell,Malden,2009.

[5]Morris E R,Walker J C.Receptor-like protein kinases:the keys to response[J].Current Opinion in Plant Biology,2003,6:339-342.

[6]Shiu S H,Karlowski W M,Pan R,et al.Comparative analysis of the receptor-like kinase family in Arabidopsis?and rice[J].Plant Cell,2004,16:1220-1234.

[7]Kobe B,Kajava A V.The leucine-rich repeat as a protein recognition motif[J].Current Opinion in Plant Biology,2001,11:725-732.

[8]Belkhadir Y,Subramaniam R,Dangl J L.Plant disease resistance protein signaling:NBS-LRR proteins and their partners[J].Current Opinion in Plant Biology,2004,7:391-399.

[9]Song W Y,Wang G L,Chen L L,et al.A receptor kinase-like protein encoded by the rice disease resistance gene Xa21[J].Science,1995,270:661-667.

[10]Wang G L,Wu C,Zeng L,et al.Isolation and characterization of rice mutants compromised in Xa21-mediated resistance to X.oryzae pv.Oryzae[J].Theoretical and Applied Genetics,2004,108:379-384.

[11]Wang Z Y,Seto H,Fujioka S,et al.BRI1 is a critical component of a plasma-membrane receptor for plant steroids[J].Nature,2001,410(6826):380-383.

[12]He Z,Wang Z Y,Li J,et al.Perception of brassinosteroids by the extracellular domain of the receptor kinase BRI1[J].Science,2000,288(3):2360-2363.

[13]Yuriko O,Kyonoshin M,Motoaki S Y,et al.Leucine-rich repeat receptor-like kinase1 is a key membrane-bound regulator of abscisic acid early signaling in Arabidopsis[J].Plant Cell,2005,17:1105-1119.

[14]Junga E H,Junga H W,Lee S C,et al.Identification of a novel pathogen-induced gene encoding a leucine-rich repeat protein expressed in phloem cells of Capsicum annuum[J].Biochimica et Biophysica Acta.2004,1676(3):211-222.

[15]ÀîÓÂ,ÖìÑÓÃ÷,Àî½Ü,µÈ.»ùÓÚLinuxµÄcDNAÎÄ¿âÐòÁзÖÎöƽ̨µÄ¹¹½¨ÓëÓ¦ÓÃ[J].ÉúÎïÐÅϢѧ,2005,3:124-127.(Li Y,Zhu Y M,Li J,et al.Construction and application of cDNA library sequence analysis platform based on Linux[J].China Journal of Bioinformatics,2005,3:124-127).

[16]ÖÜÒ˾ý,·ë½ð³¯,ÂíÎÄÎÄ,µÈ.Ö²ÎÄæ·Ö×Ó»úÖÆÑо¿½øÕ¹[J].ÖÐÑëÃñ×å´óѧѧ±¨(×ÔÈ»¿Æѧ°æ),2006,15(2):169-176.(Zhou Y J,Feng J C,Ma W W,et al.Progress of molecular mechanism of stress resistance in plants[J].Journal of the Central University for Nationalities(Natural Sciences Edition),2006,15(2):169-176).

[17]ÑîÏþ»Û,½¯ÎÀ½Ü,κçë,µÈ.Ìá¸ßÖ²ÎÑÎÄÜÁ¦µÄ¼¼Êõ´ëÊ©×ÛÊö[J].Öйúũѧͨ±¨,2006,22(1):88-91.(Yang X H,Jiang W J,Wei M,et al.The technical approaches of Improving the plant salt-resistant ability[J].Chinese Agricultural Science Bulletin,2006,22(1):88-91).

[18]¸ðçø.Ò°´ó¶¹¼îвÆÈת¼Æ×Óë»ùÒò×éÕûºÏ·ÖÎö[D].¹þ¶û±õ£º¶«±±Å©Òµ´óѧ,2010.(Ge Y.Integration of microarray and genome analysis in alkaline-stressed ª«Glycine sojaª«[D].Harbin:Northeast Agricultural University,2010).

[19]Öܹú°².´ó¶¹¿¹Äæ»ùÒòª«GmUBCª«2¡¢ª«GmPKª«ºÍª«GmNHXª«2·ÖÀëÓ빦ÄÜÑо¿[D].±±¾©:ÖйúÅ©Òµ¿ÆѧԺ,2009.(Zhou G A.Isolation and function analysis of stress-responsive genes ª«GmUBCª«2,ª«GmPKª«and ª«GmNHXª«2 from soybean(ª«Glycine Maxª«L.Merr.)[D].Beijing:Chinese Academy of Agricultural Sciences,2009).

[20]Hwang S G,Kim D S,Jang C S.Comparative analysis of evolutionary dynamics of genes encoding leucine-rich repeat receptor-like kinase between rice and ª«Arabidopsis ª«[J].Genetica,2011,139(8):1023-1032.

[21]Huang C F,Yamaji N,Ono K,et al.A leucine-rich repeat receptor-like kinase gene is involved in the specification of outer cell layers in rice roots[J].The Plant Journal,2012,69(4):565-576.

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